Hasil latihan bebas 2 MotoGP Italia 2018, sirkuit Mugello Jumat 1 Juni 2018. Andrea Iannone kembali menjadi pembalap tercepat dengan catatan waktu 1 menit 46.735 detik, unggul 0.387 detik dari Maverick Vinales yang berada di posisi kedua.
Sedangkan diposisi ketiga ditempati oleh pembalap dari tim Repsol Honda Marc Marquez yang diikuti oleh pembalap dari tim Monster Yamaha Tech 3 Johann Zarco di posisi keempat.
| Pos. | Num. | Pembalap | Negara | Tim | Motor | Km/h | Waktu | Gap 1st/Prev. |
| 1 | 29 | Andrea IANNONE | ITA | Team SUZUKI ECSTAR | Suzuki | 342.9 | 1’46.735 | |
| 2 | 25 | Maverick VIÑALES | SPA | Movistar Yamaha MotoGP | Yamaha | 346.3 | 1’47.122 | 0.387 / 0.387 |
| 3 | 93 | Marc MARQUEZ | SPA | Repsol Honda Team | Honda | 348.1 | 1’47.218 | 0.483 / 0.096 |
| 4 | 5 | Johann ZARCO | FRA | Monster Yamaha Tech 3 | Yamaha | 344.1 | 1’47.365 | 0.630 / 0.147 |
| 5 | 43 | Jack MILLER | AUS | Alma Pramac Racing | Ducati | 341.3 | 1’47.403 | 0.668 / 0.038 |
| 6 | 35 | Cal CRUTCHLOW | GBR | LCR Honda CASTROL | Honda | 350.9 | 1’47.433 | 0.698 / 0.030 |
| 7 | 46 | Valentino ROSSI | ITA | Movistar Yamaha MotoGP | Yamaha | 342.8 | 1’47.445 | 0.710 / 0.012 |
| 8 | 21 | Franco MORBIDELLI | ITA | EG 0,0 Marc VDS | Honda | 344 | 1’47.497 | 0.762 / 0.052 |
| 9 | 9 | Danilo PETRUCCI | ITA | Alma Pramac Racing | Ducati | 351.4 | 1’47.569 | 0.834 / 0.072 |
| 10 | 99 | Jorge LORENZO | SPA | Ducati Team | Ducati | 345.2 | 1’47.691 | 0.956 / 0.122 |
| 11 | 30 | Takaaki NAKAGAMI | JPN | LCR Honda IDEMITSU | Honda | 343.4 | 1’47.901 | 1.166 / 0.210 |
| 12 | 4 | Andrea DOVIZIOSO | ITA | Ducati Team | Ducati | 356.4 | 1’47.902 | 1.167 / 0.001 |
| 13 | 26 | Dani PEDROSA | SPA | Repsol Honda Team | Honda | 346.4 | 1’47.924 | 1.189 / 0.022 |
| 14 | 55 | Hafizh SYAHRIN | MAL | Monster Yamaha Tech 3 | Yamaha | 340.9 | 1’47.946 | 1.211 / 0.022 |
| 15 | 42 | Alex RINS | SPA | Team SUZUKI ECSTAR | Suzuki | 343.6 | 1’47.996 | 1.261 / 0.050 |
| 16 | 41 | Aleix ESPARGARO | SPA | Aprilia Racing Team Gresini | Aprilia | 344 | 1’48.113 | 1.378 / 0.117 |
| 17 | 53 | Tito RABAT | SPA | Reale Avintia Racing | Ducati | 346.5 | 1’48.221 | 1.486 / 0.108 |
| 18 | 44 | Pol ESPARGARO | SPA | Red Bull KTM Factory Racing | KTM | 347.4 | 1’48.269 | 1.534 / 0.048 |
| 19 | 45 | Scott REDDING | GBR | Aprilia Racing Team Gresini | Aprilia | 343.9 | 1’48.458 | 1.723 / 0.189 |
| 20 | 19 | Alvaro BAUTISTA | SPA | Angel Nieto Team | Ducati | 345 | 1’48.524 | 1.789 / 0.066 |
| 21 | 17 | Karel ABRAHAM | CZE | Angel Nieto Team | Ducati | 345 | 1’48.926 | 2.191 / 0.402 |
| 22 | 38 | Bradley SMITH | GBR | Red Bull KTM Factory Racing | KTM | 344.4 | 1’49.044 | 2.309 / 0.118 |
| 23 | 12 | Thomas LUTHI | SWI | EG 0,0 Marc VDS | Honda | 338.9 | 1’49.082 | 2.347 / 0.038 |
| 24 | 51 | Michele PIRRO | ITA | Ducati Team | Ducati | 355.2 | 1’49.111 | 2.376 / 0.029 |
| 25 | 10 | Xavier SIMEON | BEL | Reale Avintia Racing | Ducati | 345.1 | 1’49.757 | 3.022 / 0.646 |
