Hasil latihan bebas 1 MotoGP Italia 2018, sirkuit Mugello Jumat 1 Juni 2018. 3 pembalap tuan rumah pada sesi latihan bebas 1 menjadi yang tercepat. Andrea Iannone dari tim Suzuki Ecstar berhasil menjadi yang tercepat, diikuti oleh 2 pembalap dari tim Ducati Michele Pirro dan Andrea Dovizioso.
| Pos. | Num. | Pembalap | Negara | Tim | Motor | Km/h | Waktu | Gap 1st/Prev. |
| 1 | 29 | Andrea IANNONE | ITA | Team SUZUKI ECSTAR | Suzuki | 343.2 | 1’47.253 | |
| 2 | 51 | Michele PIRRO | ITA | Ducati Team | Ducati | 354.7 | 1’47.803 | 0.550 / 0.550 |
| 3 | 4 | Andrea DOVIZIOSO | ITA | Ducati Team | Ducati | 353.4 | 1’47.961 | 0.708 / 0.158 |
| 4 | 25 | Maverick VIÑALES | SPA | Movistar Yamaha MotoGP | Yamaha | 344.8 | 1’47.995 | 0.742 / 0.034 |
| 5 | 43 | Jack MILLER | AUS | Alma Pramac Racing | Ducati | 342.5 | 1’48.035 | 0.782 / 0.040 |
| 6 | 93 | Marc MARQUEZ | SPA | Repsol Honda Team | Honda | 344.8 | 1’48.035 | 0.782 |
| 7 | 21 | Franco MORBIDELLI | ITA | EG 0,0 Marc VDS | Honda | 340.6 | 1’48.211 | 0.958 / 0.176 |
| 8 | 46 | Valentino ROSSI | ITA | Movistar Yamaha MotoGP | Yamaha | 343.2 | 1’48.218 | 0.965 / 0.007 |
| 9 | 99 | Jorge LORENZO | SPA | Ducati Team | Ducati | 343.9 | 1’48.219 | 0.966 / 0.001 |
| 10 | 35 | Cal CRUTCHLOW | GBR | LCR Honda CASTROL | Honda | 347.2 | 1’48.229 | 0.976 / 0.010 |
| 11 | 5 | Johann ZARCO | FRA | Monster Yamaha Tech 3 | Yamaha | 341.7 | 1’48.272 | 1.019 / 0.043 |
| 12 | 42 | Alex RINS | SPA | Team SUZUKI ECSTAR | Suzuki | 342.3 | 1’48.274 | 1.021 / 0.002 |
| 13 | 9 | Danilo PETRUCCI | ITA | Alma Pramac Racing | Ducati | 352.2 | 1’48.304 | 1.051 / 0.030 |
| 14 | 41 | Aleix ESPARGARO | SPA | Aprilia Racing Team Gresini | Aprilia | 342.5 | 1’48.384 | 1.131 / 0.080 |
| 15 | 44 | Pol ESPARGARO | SPA | Red Bull KTM Factory Racing | KTM | 344.3 | 1’48.570 | 1.317 / 0.186 |
| 16 | 17 | Karel ABRAHAM | CZE | Angel Nieto Team | Ducati | 348.8 | 1’48.573 | 1.320 / 0.003 |
| 17 | 26 | Dani PEDROSA | SPA | Repsol Honda Team | Honda | 344.3 | 1’48.669 | 1.416 / 0.096 |
| 18 | 30 | Takaaki NAKAGAMI | JPN | LCR Honda IDEMITSU | Honda | 340 | 1’48.878 | 1.625 / 0.209 |
| 19 | 19 | Alvaro BAUTISTA | SPA | Angel Nieto Team | Ducati | 345.2 | 1’48.920 | 1.667 / 0.042 |
| 20 | 38 | Bradley SMITH | GBR | Red Bull KTM Factory Racing | KTM | 343.7 | 1’49.086 | 1.833 / 0.166 |
| 21 | 53 | Tito RABAT | SPA | Reale Avintia Racing | Ducati | 345.9 | 1’49.181 | 1.928 / 0.095 |
| 22 | 12 | Thomas LUTHI | SWI | EG 0,0 Marc VDS | Honda | 333.8 | 1’49.460 | 2.207 / 0.279 |
| 23 | 45 | Scott REDDING | GBR | Aprilia Racing Team Gresini | Aprilia | 340 | 1’49.499 | 2.246 / 0.039 |
| 24 | 55 | Hafizh SYAHRIN | MAL | Monster Yamaha Tech 3 | Yamaha | 336.2 | 1’49.650 | 2.397 / 0.151 |
| 25 | 10 | Xavier SIMEON | BEL | Reale Avintia Racing | Ducati | 345.8 | 1’50.315 | 3.062 / 0.665 |
