Hasil Latihan Bebas 3 MotoGP Italia 2018, sirkuit Mugello 2 Juni 2018. Marc Marquez berhasil menjadi yang tercepat pada sesi FP3, Marquez mencatatkan waktu 1 menit 46.439 detik, diikuti oleh Valentino Rossi yang terpaut 0.336 detik.
Sementara itu pembalap dari tim Ducati Jorge Lorenzo meraih posisi ketiga yang berada di depan Andrea Iannone.
| Pos. | Num. | Pembalap | Negara | Tim | Motor | Km/h | Waktu | Gap 1st/Prev. |
| 1 | 93 | Marc MARQUEZ | SPA | Repsol Honda Team | Honda | 343.6 | 1’46.439 | |
| 2 | 46 | Valentino ROSSI | ITA | Movistar Yamaha MotoGP | Yamaha | 339.5 | 1’46.775 | 0.336 / 0.336 |
| 3 | 99 | Jorge LORENZO | SPA | Ducati Team | Ducati | 346.4 | 1’46.782 | 0.343 / 0.007 |
| 4 | 29 | Andrea IANNONE | ITA | Team SUZUKI ECSTAR | Suzuki | 348.7 | 1’46.790 | 0.351 / 0.008 |
| 5 | 5 | Johann ZARCO | FRA | Monster Yamaha Tech 3 | Yamaha | 339.8 | 1’46.863 | 0.424 / 0.073 |
| 6 | 9 | Danilo PETRUCCI | ITA | Alma Pramac Racing | Ducati | 351.9 | 1’46.946 | 0.507 / 0.083 |
| 7 | 35 | Cal CRUTCHLOW | GBR | LCR Honda CASTROL | Honda | 346.4 | 1’46.946 | 0.507 |
| 8 | 21 | Franco MORBIDELLI | ITA | EG 0,0 Marc VDS | Honda | 341.1 | 1’46.987 | 0.548 / 0.041 |
| 9 | 42 | Alex RINS | SPA | Team SUZUKI ECSTAR | Suzuki | 343.1 | 1’47.056 | 0.617 / 0.069 |
| 10 | 4 | Andrea DOVIZIOSO | ITA | Ducati Team | Ducati | 352.1 | 1’47.086 | 0.647 / 0.030 |
| 11 | 43 | Jack MILLER | AUS | Alma Pramac Racing | Ducati | 342 | 1’47.134 | 0.695 / 0.048 |
| 12 | 26 | Dani PEDROSA | SPA | Repsol Honda Team | Honda | 347.2 | 1’47.227 | 0.788 / 0.093 |
| 13 | 41 | Aleix ESPARGARO | SPA | Aprilia Racing Team Gresini | Aprilia | 344.4 | 1’47.248 | 0.809 / 0.021 |
| 14 | 25 | Maverick VIÑALES | SPA | Movistar Yamaha MotoGP | Yamaha | 344 | 1’47.249 | 0.810 / 0.001 |
| 15 | 30 | Takaaki NAKAGAMI | JPN | LCR Honda IDEMITSU | Honda | 340.5 | 1’47.257 | 0.818 / 0.008 |
| 16 | 55 | Hafizh SYAHRIN | MAL | Monster Yamaha Tech 3 | Yamaha | 338.4 | 1’47.269 | 0.830 / 0.012 |
| 17 | 53 | Tito RABAT | SPA | Reale Avintia Racing | Ducati | 342.2 | 1’47.460 | 1.021 / 0.191 |
| 18 | 17 | Karel ABRAHAM | CZE | Angel Nieto Team | Ducati | 345.9 | 1’48.019 | 1.580 / 0.559 |
| 19 | 44 | Pol ESPARGARO | SPA | Red Bull KTM Factory Racing | KTM | 342.7 | 1’48.093 | 1.654 / 0.074 |
| 20 | 45 | Scott REDDING | GBR | Aprilia Racing Team Gresini | Aprilia | 342.9 | 1’48.148 | 1.709 / 0.055 |
| 21 | 19 | Alvaro BAUTISTA | SPA | Angel Nieto Team | Ducati | 348.8 | 1’48.194 | 1.755 / 0.046 |
| 22 | 38 | Bradley SMITH | GBR | Red Bull KTM Factory Racing | KTM | 345 | 1’48.534 | 2.095 / 0.340 |
| 23 | 12 | Thomas LUTHI | SWI | EG 0,0 Marc VDS | Honda | 335.1 | 1’48.799 | 2.360 / 0.265 |
| 24 | 10 | Xavier SIMEON | BEL | Reale Avintia Racing | Ducati | 342 | 1’49.224 | 2.785 / 0.425 |
| 51 | Michele PIRRO | ITA | Ducati Team | Ducati |