Hasil Kualifikasi MotoGP Italia 2018, Valentino Rossi berhasil meraih pole position setelah menjadi yang tercepat dengan catatan waktu 1 menit 46.208 detik dengan hasil ini Rossi menorehkan pole ke-56 dalam karirnya.
Posisi kedua diraih oleh pebalap dari tim Ducati Jorge Lorenzo yang tertinggal 0.035 detik dari Rossi, sedangkan untuk posisi grid depan terakhir diisi oleh rekan satu tim Rossi di Movistar Yamaha MotoGP Maverick Vinales yang sebelumnya berjuang untuk lolos dari kualifikasi 1.
Kualifikasi 1 MotoGP Italia 2018 :
| Pos. | Num. | Pembalap | Negara | Tim | Motor | Km/h | Waktu | Gap 1st/Prev. |
| 1 | 43 | Jack MILLER | AUS | Alma Pramac Racing | Ducati | 342.9 | 1’46.771 | |
| 2 | 25 | Maverick VIÑALES | SPA | Movistar Yamaha MotoGP | Yamaha | 346.2 | 1’46.806 | 0.035 / 0.035 |
| 3 | 53 | Tito RABAT | SPA | Reale Avintia Racing | Ducati | 349.7 | 1’46.908 | 0.137 / 0.102 |
| 4 | 55 | Hafizh SYAHRIN | MAL | Monster Yamaha Tech 3 | Yamaha | 339.9 | 1’47.188 | 0.417 / 0.280 |
| 5 | 44 | Pol ESPARGARO | SPA | Red Bull KTM Factory Racing | KTM | 344.2 | 1’47.335 | 0.564 / 0.147 |
| 6 | 19 | Alvaro BAUTISTA | SPA | Angel Nieto Team | Ducati | 346.2 | 1’47.708 | 0.937 / 0.373 |
| 7 | 38 | Bradley SMITH | GBR | Red Bull KTM Factory Racing | KTM | 345.8 | 1’47.818 | 1.047 / 0.110 |
| 8 | 30 | Takaaki NAKAGAMI | JPN | LCR Honda IDEMITSU | Honda | 334.9 | 1’47.868 | 1.097 / 0.050 |
| 9 | 12 | Thomas LUTHI | SWI | EG 0,0 Marc VDS | Honda | 337.3 | 1’47.989 | 1.218 / 0.121 |
| 10 | 26 | Dani PEDROSA | SPA | Repsol Honda Team | Honda | 343.5 | 1’48.065 | 1.294 / 0.076 |
| 11 | 41 | Aleix ESPARGARO | SPA | Aprilia Racing Team Gresini | Aprilia | 341.8 | 1’48.286 | 1.515 / 0.221 |
| 12 | 17 | Karel ABRAHAM | CZE | Angel Nieto Team | Ducati | 348.2 | 1’48.532 | 1.761 / 0.246 |
| 13 | 45 | Scott REDDING | GBR | Aprilia Racing Team Gresini | Aprilia | 346.2 | 1’48.744 | 1.973 / 0.212 |
| 14 | 10 | Xavier SIMEON | BEL | Reale Avintia Racing | Ducati | 342.2 | 1’48.794 | 2.023 / 0.050 |
Kualifikasi 1 MotoGP Italia 2018 :
| Pos. | Num. | Pembalap | Negara | Tim | Motor | Km/h | Waktu | Gap 1st/Prev. |
| 1 | 46 | Valentino ROSSI | ITA | Movistar Yamaha MotoGP | Yamaha | 343.8 | 1’46.208 | |
| 2 | 99 | Jorge LORENZO | SPA | Ducati Team | Ducati | 344 | 1’46.243 | 0.035 / 0.035 |
| 3 | 25 | Maverick VIÑALES | SPA | Movistar Yamaha MotoGP | Yamaha | 344.6 | 1’46.304 | 0.096 / 0.061 |
| 4 | 29 | Andrea IANNONE | ITA | Team SUZUKI ECSTAR | Suzuki | 344.4 | 1’46.347 | 0.139 / 0.043 |
| 5 | 9 | Danilo PETRUCCI | ITA | Alma Pramac Racing | Ducati | 350.6 | 1’46.445 | 0.237 / 0.098 |
| 6 | 93 | Marc MARQUEZ | SPA | Repsol Honda Team | Honda | 345.6 | 1’46.454 | 0.246 / 0.009 |
| 7 | 4 | Andrea DOVIZIOSO | ITA | Ducati Team | Ducati | 350.3 | 1’46.500 | 0.292 / 0.046 |
| 8 | 35 | Cal CRUTCHLOW | GBR | LCR Honda CASTROL | Honda | 347.2 | 1’46.813 | 0.605 / 0.313 |
| 9 | 5 | Johann ZARCO | FRA | Monster Yamaha Tech 3 | Yamaha | 342.7 | 1’46.830 | 0.622 / 0.017 |
| 10 | 42 | Alex RINS | SPA | Team SUZUKI ECSTAR | Suzuki | 341.3 | 1’46.909 | 0.701 / 0.079 |
| 11 | 43 | Jack MILLER | AUS | Alma Pramac Racing | Ducati | 344.8 | 1’46.998 | 0.790 / 0.089 |
| 12 | 21 | Franco MORBIDELLI | ITA | EG 0,0 Marc VDS | Honda | 343.8 | 1’47.002 | 0.794 / 0.004 |