Hasil Latihan Bebas 2 MotoGP Brno Ceko 2018, Jumat 3 Agustus 2018 :
| Pos. | Num. | Pembalap | Negara | Tim | Motor | Km/h | Waktu | Gap 1st/Prev. |
| 1 | 26 | Dani PEDROSA | SPA | Repsol Honda Team | Honda | 311.4 | 1’55.976 | |
| 2 | 9 | Danilo PETRUCCI | ITA | Alma Pramac Racing | Ducati | 310.4 | 1’56.099 | 0.123 / 0.123 |
| 3 | 19 | Alvaro BAUTISTA | SPA | Angel Nieto Team | Ducati | 308.8 | 1’56.144 | 0.168 / 0.045 |
| 4 | 55 | Hafizh SYAHRIN | MAL | Monster Yamaha Tech 3 | Yamaha | 308.2 | 1’56.200 | 0.224 / 0.056 |
| 5 | 4 | Andrea DOVIZIOSO | ITA | Ducati Team | Ducati | 311.6 | 1’56.218 | 0.242 / 0.018 |
| 6 | 25 | Maverick VIÑALES | SPA | Movistar Yamaha MotoGP | Yamaha | 309.3 | 1’56.392 | 0.416 / 0.174 |
| 7 | 46 | Valentino ROSSI | ITA | Movistar Yamaha MotoGP | Yamaha | 306.8 | 1’56.480 | 0.504 / 0.088 |
| 8 | 53 | Tito RABAT | SPA | Reale Avintia Racing | Ducati | 313 | 1’56.521 | 0.545 / 0.041 |
| 9 | 5 | Johann ZARCO | FRA | Monster Yamaha Tech 3 | Yamaha | 307.4 | 1’56.535 | 0.559 / 0.014 |
| 10 | 93 | Marc MARQUEZ | SPA | Repsol Honda Team | Honda | 309.6 | 1’56.635 | 0.659 / 0.100 |
| 11 | 29 | Andrea IANNONE | ITA | Team SUZUKI ECSTAR | Suzuki | 306 | 1’56.644 | 0.668 / 0.009 |
| 12 | 42 | Alex RINS | SPA | Team SUZUKI ECSTAR | Suzuki | 309.3 | 1’56.661 | 0.685 / 0.017 |
| 13 | 43 | Jack MILLER | AUS | Alma Pramac Racing | Ducati | 309.9 | 1’56.915 | 0.939 / 0.254 |
| 14 | 35 | Cal CRUTCHLOW | GBR | LCR Honda CASTROL | Honda | 311.6 | 1’57.014 | 1.038 / 0.099 |
| 15 | 99 | Jorge LORENZO | SPA | Ducati Team | Ducati | 310.8 | 1’57.205 | 1.229 / 0.191 |
| 16 | 41 | Aleix ESPARGARO | SPA | Aprilia Racing Team Gresini | Aprilia | 307.7 | 1’57.417 | 1.441 / 0.212 |
| 17 | 44 | Pol ESPARGARO | SPA | Red Bull KTM Factory Racing | KTM | 310.1 | 1’57.473 | 1.497 / 0.056 |
| 18 | 6 | Stefan BRADL | GER | HRC Honda Team | Honda | 310.2 | 1’57.479 | 1.503 / 0.006 |
| 19 | 21 | Franco MORBIDELLI | ITA | EG 0,0 Marc VDS | Honda | 306.1 | 1’57.498 | 1.522 / 0.019 |
| 20 | 38 | Bradley SMITH | GBR | Red Bull KTM Factory Racing | KTM | 308.9 | 1’57.506 | 1.530 / 0.008 |
| 21 | 30 | Takaaki NAKAGAMI | JPN | LCR Honda IDEMITSU | Honda | 306.9 | 1’57.647 | 1.671 / 0.141 |
| 22 | 12 | Thomas LUTHI | SWI | EG 0,0 Marc VDS | Honda | 304.8 | 1’57.729 | 1.753 / 0.082 |
| 23 | 45 | Scott REDDING | GBR | Aprilia Racing Team Gresini | Aprilia | 303.7 | 1’57.941 | 1.965 / 0.212 |
| 24 | 17 | Karel ABRAHAM | CZE | Angel Nieto Team | Ducati | 309.4 | 1’58.127 | 2.151 / 0.186 |
| 25 | 10 | Xavier SIMEON | BEL | Reale Avintia Racing | Ducati | 308 | 1’58.453 | 2.477 / 0.326 |
| 26 | 50 | Sylvain GUINTOLI | FRA | Team SUZUKI ECSTAR | Suzuki | 307.5 | 1’58.617 | 2.641 / 0.164 |
