Hasil Latihan Bebas 1 MotoGP Brno Ceko 2018, Jumat 3 Agustus 2018 :
| Pos. | Num. | Pembalap | Negara | Tim | Motor | Km/h | Waktu | Gap 1st/Prev. |
| 1 | 5 | Johann ZARCO | FRA | Monster Yamaha Tech 3 | Yamaha | 305.4 | 1’56.647 | |
| 2 | 4 | Andrea DOVIZIOSO | ITA | Ducati Team | Ducati | 314 | 1’56.720 | 0.073 / 0.073 |
| 3 | 93 | Marc MARQUEZ | SPA | Repsol Honda Team | Honda | 307.5 | 1’56.828 | 0.181 / 0.108 |
| 4 | 19 | Alvaro BAUTISTA | SPA | Angel Nieto Team | Ducati | 306.2 | 1’56.831 | 0.184 / 0.003 |
| 5 | 46 | Valentino ROSSI | ITA | Movistar Yamaha MotoGP | Yamaha | 306.9 | 1’56.888 | 0.241 / 0.057 |
| 6 | 29 | Andrea IANNONE | ITA | Team SUZUKI ECSTAR | Suzuki | 305.4 | 1’57.079 | 0.432 / 0.191 |
| 7 | 26 | Dani PEDROSA | SPA | Repsol Honda Team | Honda | 309.4 | 1’57.289 | 0.642 / 0.210 |
| 8 | 6 | Stefan BRADL | GER | HRC Honda Team | Honda | 311.8 | 1’57.291 | 0.644 / 0.002 |
| 9 | 43 | Jack MILLER | AUS | Alma Pramac Racing | Ducati | 305.7 | 1’57.321 | 0.674 / 0.030 |
| 10 | 53 | Tito RABAT | SPA | Reale Avintia Racing | Ducati | 310.7 | 1’57.325 | 0.678 / 0.004 |
| 11 | 35 | Cal CRUTCHLOW | GBR | LCR Honda CASTROL | Honda | 310.8 | 1’57.386 | 0.739 / 0.061 |
| 12 | 99 | Jorge LORENZO | SPA | Ducati Team | Ducati | 307.2 | 1’57.426 | 0.779 / 0.040 |
| 13 | 55 | Hafizh SYAHRIN | MAL | Monster Yamaha Tech 3 | Yamaha | 305.5 | 1’57.428 | 0.781 / 0.002 |
| 14 | 25 | Maverick VIÑALES | SPA | Movistar Yamaha MotoGP | Yamaha | 308.3 | 1’57.764 | 1.117 / 0.336 |
| 15 | 9 | Danilo PETRUCCI | ITA | Alma Pramac Racing | Ducati | 311.5 | 1’57.938 | 1.291 / 0.174 |
| 16 | 30 | Takaaki NAKAGAMI | JPN | LCR Honda IDEMITSU | Honda | 304.6 | 1’58.027 | 1.380 / 0.089 |
| 17 | 42 | Alex RINS | SPA | Team SUZUKI ECSTAR | Suzuki | 305.4 | 1’58.032 | 1.385 / 0.005 |
| 18 | 44 | Pol ESPARGARO | SPA | Red Bull KTM Factory Racing | KTM | 309.6 | 1’58.098 | 1.451 / 0.066 |
| 19 | 50 | Sylvain GUINTOLI | FRA | Team SUZUKI ECSTAR | Suzuki | 305.1 | 1’58.114 | 1.467 / 0.016 |
| 20 | 12 | Thomas LUTHI | SWI | EG 0,0 Marc VDS | Honda | 302.9 | 1’58.226 | 1.579 / 0.112 |
| 21 | 38 | Bradley SMITH | GBR | Red Bull KTM Factory Racing | KTM | 305.5 | 1’58.238 | 1.591 / 0.012 |
| 22 | 21 | Franco MORBIDELLI | ITA | EG 0,0 Marc VDS | Honda | 303.1 | 1’58.299 | 1.652 / 0.061 |
| 23 | 45 | Scott REDDING | GBR | Aprilia Racing Team Gresini | Aprilia | 303.2 | 1’58.497 | 1.850 / 0.198 |
| 24 | 41 | Aleix ESPARGARO | SPA | Aprilia Racing Team Gresini | Aprilia | 305 | 1’58.635 | 1.988 / 0.138 |
| 25 | 17 | Karel ABRAHAM | CZE | Angel Nieto Team | Ducati | 306.4 | 1’59.052 | 2.405 / 0.417 |
| 26 | 10 | Xavier SIMEON | BEL | Reale Avintia Racing | Ducati | 304.9 | 1’59.863 | 3.216 / 0.811 |
